cholesteryl ester fluorometric assay kit  (Elabscience Biotechnology)

Journal: Chinese Medicine

Article Title: Explore the potential mechanism of Huachansu injection against osteosarcoma via metabolomics, network pharmacology and bioinformatics

doi: 10.1186/s13020-025-01179-x

Figure Lengend Snippet: HCSI regulated lipid metabolism in osteosarcoma cells. A - B Observation of neutral lipid content by staining with fluorescence dye BODIPY 493/503 in U2OS and SAOS2 cells with HCSI treatment. C Quantitative RT-PCR for mRNA expression levels of SCD1, SREBF1, HMGCR, HMGCS1 and CD36 with HCSI treatment (n = 4). D - E Protein levels of SCD1, SREBF1, HMGCR in H U2OS and SAOS2 cells with HCSI treatment. F Spearman correlation analysis of the relationship between the mRNA expression levels of HMGCR and SCD1, HMGCS1 and HMGCR in tumor tissues from 42 osteosarcoma patients. G Intracellular levels of cholesterol were measured in U2OS and SAOS2 cells with HCSI treatment. H - I Observation of intracellular ROS levels by staining with fluorescence dye DCFH-DA in U2OS and SAOS2 cells with HCSI treatment. Data are presented as mean ± SD (n = 3). *P < 0.05, **P < 0.01, and ***P < 0.001 versus the Control group

Article Snippet: Total Cholesterol and Cholesteryl Ester Fluorometric Assay Kit was ordered from Elabscience (Wuhan, China).

Techniques: Staining, Fluorescence, Quantitative RT-PCR, Expressing, Control

Journal: Chinese Medicine

Article Title: Explore the potential mechanism of Huachansu injection against osteosarcoma via metabolomics, network pharmacology and bioinformatics

doi: 10.1186/s13020-025-01179-x

Figure Lengend Snippet: HCSI regulated lipid metabolism in osteosarcoma cells. A Intracellular levels of cholesterol were measured in U2OS and SAOS2 cells with HCSI and simvastatin treatment. B - D Quantitative RT-PCR for mRNA, miRNA and lncRNA expression levels of BCYRN1, miR-27a-3p and HMGCR with HCSI and simvastatin treatment. (n = 4). E – F Protein levels of SCD1, SREBF1, HMGCR in the U2OS and SAOS2 cells with HCSI and simvastatin treatment. G - H Flow cytometry detected that HCSI and simvastatin induces apoptosis in U2OS and SAOS2 cells. I - J Western blotting was used to detect the expression of proliferative protein Bcl-2 and Bax after 24 h of intervention with HCSI and simvastatin treatment. Data are presented as mean ± SD (n = 3). *P < 0.05, **P < 0.01, and ***P < 0.001 versus the Control group

Article Snippet: Total Cholesterol and Cholesteryl Ester Fluorometric Assay Kit was ordered from Elabscience (Wuhan, China).

Techniques: Quantitative RT-PCR, Expressing, Flow Cytometry, Western Blot, Control

Journal: BMC Biology

Article Title: Disturbed intracellular folate homeostasis impairs autophagic flux and increases hepatocytic lipid accumulation

doi: 10.1186/s12915-024-01946-6

Figure Lengend Snippet: Increased lipid accumulation was found in FD zebrafish liver. The transgenic larvae and adult fish were induced for FD and examined for their hepatic lipid deposition. a The liver of larvae of 11 dpf and adult fish after completing FD-induction protocols were collected and subjected to microbiology assay. Data were collected from 6 and 4 independent experiments for larval liver and adult fish liver, respectively. b - c The cryosections prepared from adult fish liver ( b ) and whole larvae ( c ) were examined for lipid deposition (black arrows) with Oil Red O staining. d - e The hepatic lipid accumulation (red arrow) in larvae at 11 dpf, with/without FA, 5-CHO-THF, and 5-CH 3 -THF supplementation, were stained with Oil Red O (red arrow) ( d ) and scored ( e ) following the criteria based on the percentage of intensely stained area occupied in larval liver: less than 30% (1), between 30% to 70% (2), or more than 70% (3). Those showed no significant staining signal was scored (0). Data were collected from at least 3 independent experiments. f - i Hepatic cholesterol ( f - g ) and triglyceride ( h - i ) in adult fish liver ( f and h ) and larvae at 11 dpf ( g and i ) were measured with colorimetric/fluorometric Assay. Significant increase was found for the triglyceride levels in both adult fish liver and larval liver. Supplementing with 5-CHO-THF effectively prevented the increase of triglyceride in FD larvae. All the data presented are the averages of at least three independent trials with 10-40 larvae or 4-10 adult fish livers for each group. CTL, control (Tg-GGH/LR larvae without FD); FD, folate deficiency; FA, folic acid; 5-CHO, 5-formyl-THF; 5-CH 3, 5-methyl-THF. Statistical data are shown in mean ± SEM. * p <0.05, **, p <0.01; ****, p <0.0001

Article Snippet: Total Cholesterol and Cholesteryl Ester Colorimetric/Fluorometric Assay Kit and Triglyceride Quantification Colorimetric/Fluorometric Kit were purchased from BioVision, Inc. (SF, USA).

Techniques: Transgenic Assay, Staining, Control

Journal: BMC Biology

Article Title: Disturbed intracellular folate homeostasis impairs autophagic flux and increases hepatocytic lipid accumulation

doi: 10.1186/s12915-024-01946-6

Figure Lengend Snippet: Increased lipid deposition was found in FD Huh7 cells. Huh7 cells were cultivated in FD medium and examined for cell viability and lipid content. a Huh7 cells cultivated in CTL (0.004 g/L folic acid) or folate-deficient (FD) medium were analyzed with microbiology assay for folate content. A more than 90% depletion of intracellular folate content was established at 10 day-post-seeding (dps) for the cells growing in FD medium. Presented are data collected from 3 and 4 independent experiments at 5 dps, and 8 and 10 dps, respectively. b Huh7 cells were cultivated in CTL or FD medium for 7 days (dps) before adding FA or 5-CHO-THF for rescue. Cell viability was examined on 8 dps. The decreased cell viability due to FD was successfully rescued by the presence of FA or 5-CHO-THF. Data were collected from at least 6 independent experiments. c Cells were stained with Nile red and analyzed for lipid content with direct visual inspection with fluorescence microscopy (left) and quantified by flow cytometry (right). Scale bars = 40 µm. Data were collected from at least 7 independent experiments. d - e The cholesterol ( d ) and triglyceride ( e ) levels of Huh7 cells at 8 dps was measured with colorimetric/fluorometric Assay. Data were collected from at least 6 independent experiments. f - g Cells cultivated in FD medium with/without FA or 5-CHO-THF supplementation were examined for ALT ( f ) and AST ( g ) content. The increase of both ALT and AST was effectively alleviated by supplementing with FA or 5-CHO-THF. Presented are the averaged results of at least 8 independent trials. CTL, control (cells without FD); FD, folate deficiency. Statistical data are shown in mean ± SEM. * p <0.05, **, p <0.01; ***, p <0.001, ****, p <0.0001

Article Snippet: Total Cholesterol and Cholesteryl Ester Colorimetric/Fluorometric Assay Kit and Triglyceride Quantification Colorimetric/Fluorometric Kit were purchased from BioVision, Inc. (SF, USA).

Techniques: Staining, Fluorescence, Microscopy, Flow Cytometry, Control

Journal: iScience

Article Title: Hepatic Huwe1 loss protects mice from non-alcoholic fatty liver disease through lipid metabolic rewiring

doi: 10.1016/j.isci.2023.108405

Figure Lengend Snippet:

Article Snippet: Hepatic triglyceride levels were measured using Infinity Triglycerides Liquid Stable Reagent (Thermo Scientific) and total cholesterol levels were measured using Total Cholesterol and Cholesteryl Ester Fluorometric Assay Kit (BioVision) according to manufacturer’s instructions.

Techniques: Recombinant, Staining, Mass Spectrometry, Software